Isolation of the Arabidopsis ABI3 gene by positional cloning.

Clicks: 2
ID: 299069
1992
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #123 of 308 articles by views in The Plant cell

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 308 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein.
Reference Key
openalex_W2141985540 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors J. Giraudat, Brian M. Hauge, Christiane Valon, Jan Smalle, François Parcy, H M Goodman
Journal The Plant cell
Year 1992
DOI
10.1105/tpc.4.10.1251
URL
https://academic.oup.com/plcell/article-pdf/4/10/1251/34806497/plcell_v4_10_1251.pdf
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.