A review of late Eocene mammalian faunas from North America

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ID: 295939
1966
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Abstract

Abstract

Cellobiose metabolism is linked to the virulence properties in numerous bacterial pathogens. Here, we characterized a putative cellobiose PTS operon of Clostridiodes difficile to investigate the role of cellobiose metabolism in C. difficile pathogenesis. Our gene knockout experiments demonstrated that the putative cellobiose operon enables uptake of cellobiose into C. difficile and allows growth when cellobiose is provided as the sole carbon source in minimal medium. Additionally, using reporter gene fusion assays and DNA pull-down experiments, we show that its transcription is regulated by CelR, a novel transcriptional repressor protein, which directly binds to the upstream region of the cellobiose operon to control its expression. We have also identified cellobiose metabolism to play a significant role in C. difficile physiology as observed by the reduction of sporulation efficiency when cellobiose uptake was compromised in the mutant strain. In corroboration to in vitro study findings, our in vivo hamster challenge experiment showed a significant reduction of pathogenicity by the cellobiose mutant strain in both the primary and the recurrent infection model- substantiating the role of cellobiose metabolism in C. difficile pathogenesis.
Reference Key
openalex_W2160940550 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Candice C. Black, Michelle Dawson
Journal american journal of science
Year 1966
DOI
10.2475/ajs.264.5.321
URL
https://doi.org/10.2475/ajs.264.5.321
Keywords Keywords not found

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