pEMBL: a new family of single stranded plasmids

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ID: 295782
1983
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Abstract
We have constructed a series of plasmids, the pEMBL family, characterized by the presence of 1) the bla gene as selectable marker, 2) a short segment coding for the alpha-peptide of beta-galactosidase and containing a multiple cloning sites polylinker, 3) the intragenic region of phage F1. pEMBL plasmids have the property of being encapsidated as single stranded DNA, upon superinfection with phage F1. These vectors have been used successfully for DNA sequencing with the dideoxy-method, and can be used for any other purpose for which M13 derivatives are used. However, the pEMBL plasmids have the advantage of being smaller than M13 vectors, and the purification of the DNA is simpler. In addition, and most importantly, long inserts have a higher stability in pEMBL plasmids than M13 vectors.
Reference Key
openalex_W2008936546 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Luciana Dente, Gianni Cesareni, Riccardo Cortese
Journal Nucleic Acids Research
Year 1983
DOI
10.1093/nar/11.6.1645
URL
https://doi.org/10.1093/nar/11.6.1645
Keywords Keywords not found

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