Mapping adenines, guanines, and pyrimidines in RNA

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ID: 294472
1977
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Abstract
The positions of adenines, guanines, and pyrimidines can be determined by partial nuclease digestion of a terminally labeles RNA molecule. In urea, at elevated temperatures, RNase T1 generates a pattern reflecting cleavage at guanines while RNase U2 cleaves only at adenine. A limited alkaline hydrolysis provides a continuum of fragments derived from breaks at every phosphodiester bond. The reaction products are electrophoretically fractionated by size in adjacent lanes of a polyacrylamide gel. An autoradiograph of the gel displays the sequence up to 100 nucleotides from the end of the molecule, although uracil cannot as yet be distinguished from cytosine. These techniques form the basis of an RNA sequencing method and are demonstrated on yeast 5.8S ribosomal RNA.
Reference Key
openalex_W1970667469 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Helen Donis-Keller, Allan M. Maxam, Walter Gilbert
Journal Nucleic Acids Research
Year 1977
DOI
10.1093/nar/4.8.2527
URL
https://doi.org/10.1093/nar/4.8.2527
Keywords Keywords not found

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