λ ZAP: a bacteriophage λ expression vector within vivoexcision properties

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ID: 294461
1988
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Abstract
A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed.In E. coli a phagemid, pBluescnpt SK(-), contained within the vector, can be excised by f1 or M13 helper phage.The excision process eliminates the need to subclone DNA inserts from the lambda phage into a plasmid by restrction digestion and ligation.This is possible because Lambda ZAP incorporates the signals for both initiation and termination of DNA synthesis from the fI bacteriophage origin of replication (1).Six of 21 restriction sites in the excised pBluescript SK polyflinker, contained within the NH2-portion of the lacZ gene, are unique in lambda ZAP.Coding sequences inserted into these restriction sites, in the appropriate reading frame, can be expressed from the lacZpromoter as fusion proteins.The features of this vector significantly increase the rate at which clones can be isolated and analyzed.The lambda ZAP vector was tested by the preparation of a chicken liver cDNA library and the isolation of actin clones by screening with oligonucleotide probes.Putative actin clones were excised from the lambda vector and identified by DNA sequencing.The ability of lambda ZAP to serve as a vector for the constnuction of cDNA expression libraries was determined by detecting fusion proteins from clones containing glucocerbrosidase cDNA's using rabbit IgG anti-glucocerbrosidase antibodies.
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openalex_W2074122548 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Jay M. Short, Joseph M. Fernandez, Joseph A. Sorge, William D. Huse
Journal Nucleic Acids Research
Year 1988
DOI
10.1093/nar/16.15.7583
URL
https://doi.org/10.1093/nar/16.15.7583
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