AUGUSTUS: a web server for gene prediction in eukaryotes that allows user-defined constraints

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ID: 292188
2005
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Abstract
We present a WWW server for AUGUSTUS, a software for gene prediction in eukaryotic genomic sequences that is based on a generalized hidden Markov model, a probabilistic model of a sequence and its gene structure. The web server allows the user to impose constraints on the predicted gene structure. A constraint can specify the position of a splice site, a translation initiation site or a stop codon. Furthermore, it is possible to specify the position of known exons and intervals that are known to be exonic or intronic sequence. The number of constraints is arbitrary and constraints can be combined in order to pin down larger parts of the predicted gene structure. The result then is the most likely gene structure that complies with all given user constraints, if such a gene structure exists. The specification of constraints is useful when part of the gene structure is known, e.g. by expressed sequence tag or protein sequence alignments, or if the user wants to change the default prediction. The web interface and the downloadable stand-alone program are available free of charge at http://augustus.gobics.de/submission.
Reference Key
openalex_W2161745371 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mario Stanke, Burkhard Morgenstern
Journal Nucleic Acids Research
Year 2005
DOI
10.1093/nar/gki458
URL
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