Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

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ID: 291977
1984
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Abstract
We describe a method for the synthesis of microgram quantities of eucaryotic messenger RNAs. Injection into the cytoplasm of frog oocytes and addition to wheat germ extracts show that these synthetic RNAs function efficiently as messenger RNAs. We confirm that a 5′ cap on the mRNA is essential for translation in injected oocytes and show that most of the 3′ flanking region, including the poly A tail, can be deleted without the abolition of protein synthesis. The method of mRNA synthesis involves invitro transcription of cDNAs which have been cloned into SP6 vectors (described in the accompanying paper). This method enables one to produce large amounts of mRNA and consequently protein from any cDNA clone.
Reference Key
openalex_W2064864270 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Paul A. Krieg, Douglas A. Melton
Journal Nucleic Acids Research
Year 1984
DOI
10.1093/nar/12.18.7057
URL
https://doi.org/10.1093/nar/12.18.7057
Keywords Keywords not found

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