Estimating Synonymous and Nonsynonymous Substitution Rates Under Realistic Evolutionary Models

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ID: 291555
2000
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Abstract
Approximate methods for estimating the numbers of synonymous and nonsynonymous substitutions between two DNA sequences involve three steps: counting of synonymous and nonsynonymous sites in the two sequences, counting of synonymous and nonsynonymous differences between the two sequences, and correcting for multiple substitutions at the same site. We examine complexities involved in those steps and propose a new approximate method that takes into account two major features of DNA sequence evolution: transition/transversion rate bias and base/codon frequency bias. We compare the new method with maximum likelihood, as well as several other approximate methods, by examining infinitely long sequences, performing computer simulations, and analyzing a real data set. The results suggest that when there are transition/transversion rate biases and base/codon frequency biases, previously described approximate methods for estimating the nonsynonymous/synonymous rate ratio may involve serious biases, and the bias can be both positive and negative. The new method is, in general, superior to earlier approximate methods and may be useful for analyzing large data sets, although maximum likelihood appears to always be the method of choice.
Reference Key
openalex_W2115011276 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ziheng Yang, Rasmus Nielsen
Journal molecular biology and evolution
Year 2000
DOI
10.1093/oxfordjournals.molbev.a026236
URL
Keywords Keywords not found

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