PCR inhibitors - occurrence, properties and removal

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ID: 291213
2012
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Abstract
The polymerase chain reaction (PCR) is increasingly used as the standard method for detection and characterization of microorganisms and genetic markers in a variety of sample types. However, the method is prone to inhibiting substances, which may be present in the analysed sample and which may affect the sensitivity of the assay or even lead to false-negative results. The PCR inhibitors represent a diverse group of substances with different properties and mechanisms of action. Some of them are predominantly found in specific types of samples thus necessitating matrix-specific protocols for preparation of nucleic acids before PCR. A variety of protocols have been developed to remove the PCR inhibitors. This review focuses on the general properties of PCR inhibitors and their occurrence in specific matrices. Strategies for their removal from the sample and for quality control by assessing their influence on the individual PCR test are presented and discussed.
Reference Key
openalex_W1973207608 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Christina Schrader, Anika Schielke, Lüppo Ellerbroek, Reimar Johne
Journal Journal of applied microbiology
Year 2012
DOI
10.1111/j.1365-2672.2012.05384.x
URL
https://doi.org/10.1111/j.1365-2672.2012.05384.x
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