A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system

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ID: 290991
1981
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Abstract
The use of gel electrophoresis for quantitative studies of DNA-protein interactions is described. This rapid and simple technique involves separation of free DNA from DNA-protein complexes based on differences in their electrophoretic mobilities in polyacrylamide gels. Under favorable conditions both unbound DNA and DNA associated with protein can be quantified.
Reference Key
openalex_W1965267927 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mark M. Garner, Arnold Revzin
Journal Nucleic Acids Research
Year 1981
DOI
10.1093/nar/9.13.3047
URL
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