A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
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ID: 290991
1981
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Abstract
The use of gel electrophoresis for quantitative studies of DNA-protein interactions is described. This rapid and simple technique involves separation of free DNA from DNA-protein complexes based on differences in their electrophoretic mobilities in polyacrylamide gels. Under favorable conditions both unbound DNA and DNA associated with protein can be quantified.
| Reference Key |
openalex_W1965267927
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|---|---|
| Authors | Mark M. Garner, Arnold Revzin |
| Journal | Nucleic Acids Research |
| Year | 1981 |
| DOI |
10.1093/nar/9.13.3047
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| URL | |
| Keywords | Keywords not found |
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