Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

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ID: 290916
2014
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Abstract
Abstract Summary: The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system is an adaptive immune response in prokaryotes, protecting host cells against invading phages or plasmids by cleaving these foreign DNA species in a targeted manner. CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) enable genome editing in cultured cells, animals and plants, but are limited by off-target mutations. Here, we present a novel algorithm termed Cas-OFFinder that searches for potential off-target sites in a given genome or user-defined sequences. Unlike other algorithms currently available for identification of RGEN off-target sites, Cas-OFFinder is not limited by the number of mismatches and allows variations in protospacer-adjacent motif sequences recognized by Cas9, the essential protein component in RGENs. Cas-OFFinder is available as a command-line program or accessible via our website. Availability and implementation: Cas-OFFinder free access at http://www.rgenome.net/cas-offinder. Contact: baesau@snu.ac.kr or jskim01@snu.ac.kr
Reference Key
openalex_W2115603949 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Sangsu Bae, Jeongbin Park, Jin‐Soo Kim
Journal BMC Bioinformatics
Year 2014
DOI
10.1093/bioinformatics/btu048
URL
https://academic.oup.com/bioinformatics/article-pdf/30/10/1473/744247/btu048.pdf
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