Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis
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ID: 290892
1997
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Abstract
After nearly 10 years of PCR-based analysis of prokaryotic small-subunit ribosomal RNAs for ecological studies it seems necessary to summarize reported pitfalls of this approach which will most likely lead to an erroneous description on the microbial diversity of a given habitat. The following article will cover specific aspects of sample collection, cell lysis, nucleic acid extraction, PCR amplification, separation of amplified DNA, application of nucleic probes and data analysis.
| Reference Key |
openalex_W2125862549
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|---|---|
| Authors | Friedrich von Wintzingerode, Ulf B. Göbel, Erko Stackebrandt |
| Journal | FEMS microbiology reviews |
| Year | 1997 |
| DOI |
10.1111/j.1574-6976.1997.tb00351.x
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| URL | |
| Keywords | Keywords not found |
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