Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)

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ID: 290414
1989
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Abstract
We have improved the "polymerase chain reaction" (PCR) to permit rapid analysis of any known mutation in genomic DNA. We demonstrate a system, ARMS (Amplification Refractory Mutation System), that allows genotyping solely by inspection of reaction mixtures after agarose gel electrophoresis. The system is simple, reliable and non-isotopic. It will clearly distinguish heterozygotes at a locus from homozygotes for either allele. The system requires neither restriction enzyme digestion, allele-specific oligonucleotides as conventionally applied, nor the sequence analysis of PCR products. The basis of the invention is that unexpectedly, oligonucleotides with a mismatched 3'-residue will not function as primers in the PCR under appropriate conditions. We have analysed DNA from patients with alpha 1-antitrypsin (AAT) deficiency, from carriers of the disease and from normal individuals. Our findings are in complete agreement with allele assignments derived by direct sequencing of PCR products.
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openalex_W2064063117 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Charles R. Newton, Alexander Graham, Lesley Heptinstall, Stephen Joseph Powell, Claire Summers, Noor Kalsheker, John Smith, Alexander F. Markham
Journal Nucleic Acids Research
Year 1989
DOI
10.1093/nar/17.7.2503
URL
https://doi.org/10.1093/nar/17.7.2503
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