A system for shotgun DNA sequencing

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ID: 290269
1981
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Abstract
A multipurpose cloning site has been introduced into the gene for B-galactosidase (s-D-galactosidegalactohydrolase, EC 3.21.23)on the single-stranded DMA phage M13mp2 (Gronenborn, B. and Messing, J., (1978) Nature ^72, 375-377) with the use of synthetic DNA.The site contributes 14 additional codons and does not affect the ability of the lac gene product to undergo intracistronic complementation.Two restriction endonuclease cleavage sites in the viral gene II ware removed by singls base-pair mutations.Using the new phage M13mp7, DNA fragments generated by cleavage with a variety of different restriction endonucleases can be cloned directly.The nucleotide sequences of the cloned DNAs can be determined rapidly by DNA synthesis using chain terminators and a synthetic oligonucleotide primer complementary to 15 bases preceeding the new array of restriction sites.
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openalex_W2037457710 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Joachim Messing, Roberto Crea, Peter H. Seeburg
Journal Nucleic Acids Research
Year 1981
DOI
10.1093/nar/9.2.309
URL
https://doi.org/10.1093/nar/9.2.309
Keywords Keywords not found

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