GeSeq – versatile and accurate annotation of organelle genomes

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ID: 290243
2017
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Abstract
We have developed the web application GeSeq (https://chlorobox.mpimp-golm.mpg.de/geseq.html) for the rapid and accurate annotation of organellar genome sequences, in particular chloroplast genomes. In contrast to existing tools, GeSeq combines batch processing with a fully customizable reference sequence selection of organellar genome records from NCBI and/or references uploaded by the user. For the annotation of chloroplast genomes, the application additionally provides an integrated database of manually curated reference sequences. GeSeq identifies genes or other feature-encoding regions by BLAT-based homology searches and additionally, by profile HMM searches for protein and rRNA coding genes and two de novo predictors for tRNA genes. These unique features enable the user to conveniently compare the annotations of different state-of-the-art methods, thus supporting high-quality annotations. The main output of GeSeq is a GenBank file that usually requires only little curation and is instantly visualized by OGDRAW. GeSeq also offers a variety of optional additional outputs that facilitate downstream analyzes, for example comparative genomic or phylogenetic studies.
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openalex_W2612261225 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Michael Tillich, Pascal Lehwark, Tommaso Pellizzer, Elena S. Ulbricht-Jones, Axel Fischer, Ralph Bock, Stephan Greiner
Journal Nucleic Acids Research
Year 2017
DOI
10.1093/nar/gkx391
URL
https://academic.oup.com/nar/article-pdf/45/W1/W6/18137544/gkx391.pdf
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