QUAST: quality assessment tool for genome assemblies

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ID: 289234
2013
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Abstract
Abstract Summary: Limitations of genome sequencing techniques have led to dozens of assembly algorithms, none of which is perfect. A number of methods for comparing assemblers have been developed, but none is yet a recognized benchmark. Further, most existing methods for comparing assemblies are only applicable to new assemblies of finished genomes; the problem of evaluating assemblies of previously unsequenced species has not been adequately considered. Here, we present QUAST—a quality assessment tool for evaluating and comparing genome assemblies. This tool improves on leading assembly comparison software with new ideas and quality metrics. QUAST can evaluate assemblies both with a reference genome, as well as without a reference. QUAST produces many reports, summary tables and plots to help scientists in their research and in their publications. In this study, we used QUAST to compare several genome assemblers on three datasets. QUAST tables and plots for all of them are available in the Supplementary Material, and interactive versions of these reports are on the QUAST website. Availability: http://bioinf.spbau.ru/quast Contact: gurevich@bioinf.spbau.ru Supplementary information: Supplementary data are available at Bioinformatics online.
Reference Key
openalex_W2107772251 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Alexey Gurevich, Vladislav Saveliev, Nikolay Vyahhi, Glenn Tesler
Journal BMC Bioinformatics
Year 2013
DOI
10.1093/bioinformatics/btt086
URL
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