Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

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ID: 289176
1983
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Abstract
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
Reference Key
openalex_W1993243592 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors John David Dignam, Russell M. Lebovitz, R G Roeder
Journal Nucleic Acids Research
Year 1983
DOI
10.1093/nar/11.5.1475
URL
https://doi.org/10.1093/nar/11.5.1475
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