BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs

Clicks: 24
ID: 289173
2015
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Ranked #674 of 991 articles by views in BMC Bioinformatics

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Abstract
Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.Software implemented in Python and datasets available for download from http://busco.ezlab.org.evgeny.zdobnov@unige.chSupplementary data are available at Bioinformatics online.
Reference Key
openalex_W2155628349 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors
Journal BMC Bioinformatics
Year 2015
DOI
10.1093/bioinformatics/btv351
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