DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

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ID: 289160
1990
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Abstract
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendellan fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
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openalex_W2171114940 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors John G. Williams, Anne R. Kubelik, Kenneth J. Livak, J. Antoni Rafalski, Scott Tingey
Journal Nucleic Acids Research
Year 1990
DOI
10.1093/nar/18.22.6531
URL
https://academic.oup.com/nar/article-pdf/18/22/6531/3601793/18-22-6531.pdf
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