A rapid alkaline extraction procedure for screening recombinant plasmid DNA

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ID: 289133
1979
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Abstract
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.
Reference Key
openalex_W2019410656 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors H.C. Birnboim, Janine Doly
Journal Nucleic Acids Research
Year 1979
DOI
10.1093/nar/7.6.1513
URL
https://doi.org/10.1093/nar/7.6.1513
Keywords Keywords not found

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