Antibacterial Effect of Ethanolic Extracts of L. Jacq. and L. against Staphylococci Isolated from Skin Lesions.

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ID: 281708
2023
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Abstract
The resistance to antibiotics shown by some dermatological pathogenic microorganisms has increased the interest of pharmaceutical and cosmetic industries in developing natural products that possess different biological activities, including antimicrobial effects. In the present investigation, the antibacterial activity of ethanolic extracts of aerial part and leaves and seed was evaluated against resistant strains of isolated from skin lesions and against ATCC 25923 (reference strain). Column chromatography (CC) and preparative thin-layer chromatography (PTLC) were used to obtain separate fractions of the seed extract of . We also determined the antimicrobial resistance of the strains against antibiotics using the agar disc diffusion assay. In addition, phytochemical screening was performed by colorimetric standard techniques. seed extract showed the highest antibacterial activity with MBC from 2.3 g/mL to 19.5 g/mL without differences with gentamicin ( = 0.998). The isolated strain I showed the highest antimicrobial resistance against the tested antibiotics. PTLC-fractions of seed extract showed MBC from 3.2 g/mL to 40.7 g/mL against I and 25923 (reference), respectively, which suggests a synergistic effect of the secondary metabolites present in the crude ethanolic extract compared to its active PTLC-fractions, where only coumarins and compounds with lactone groups were detected in the phytochemical screening. seed extract has promising effects that should be considered in further studies as an alternative or adjuvant in treating skin infections caused by staphylococci.
Reference Key
pérez-narváez2023antibacterial Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pérez-Narváez, Oscar Alberto; Castillo Hernández S, Sandra Loruhama; Leos-Rivas, Catalina; Pérez-Hernández, Raymundo Alejandro; Chávez-Montes, Abelardo; Verduzco-Martínez, Jorge Armando; Sánchez-García, Eduardo
Journal BioMed research international
Year 2023
DOI
10.1155/2023/5584412
URL
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