A magnetic bifunctional endotoxin removal nano-agent for the efficient elimination of endotoxins in recombinant protein preparation.

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ID: 281399
2025
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Abstract
Endotoxin contamination, as one of the most significant challenges in recombinant protein production by Escherichia coli, represents a critical biosafety concern and greatly hinders the biomedical application of recombinant proteins. Conventional methods, such as extreme-condition inactivation and chromatography-based separation, are plagued by issues including protein denaturation, low efficiency, and operational complexity in endotoxin removal. In this work, we developed a novel magnetic bifunctional endotoxin removal nano-agent (MagBER) with a multi-layered structure, consisting of a superparamagnetic FeO core, a mesoporous TiO intermediary layer, and an outer shell functionalized with boronic acid groups. This dual-functional design significantly enhances endotoxin removal efficiency through the TiO layer and boronic acid groups, ensuring stable endotoxin clearance performance even in high-salt environments and complex biological matrices. MagBER exhibits reusability while maintaining protein structural integrity. Moreover, MagBER has been successfully employed for endotoxin removal in various proteins, establishing it as a promising and sustainable solution for endotoxin clearance in biopharmaceutical applications.
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zeng2025ainternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zeng, Zibing;Wei, Nannan;Cai, Xiangdong;Xiao, Jianxi;
Journal International journal of biological macromolecules
Year 2025
DOI
10.1016/j.ijbiomac.2025.143663
URL
Keywords
recombinant protein magnetic nanomaterial endotoxin removal

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