Rapid cell counting and viability detection method of Escherichia coli Nissle using image cytometry.

Clicks: 267
ID: 274793
2021
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Abstract
The improvement of the cell enumeration methods for cell counting Escherichia coli (E. coli) is important as E. coli gains in popularity as a basis for biopharmaceutical applications. In the biopharmaceutical industry, enumerating, characterizing, and dosing the accurate number of cells is imperative. In this work, we demonstrate the utilization of a chip-based image cytometer using a thin-gap, low volume counting chamber consumable to directly enumerate E. coli in bright field and fluorescence, and measure their viability using SYTOX™ Green. The total E. coli particles can be counted accurately label-free by adjusting the focus and targeting the linear range of the instrument. The E. coli are stained with SYTOX™ Green to count the membrane-compromised dead bacterial cells in the green fluorescence channel, while the total cells are counted using the bright field channel. Optimization of system settings, image focus, cell counting range, and staining conditions have yielded a precise, rapid, and accurate E. coli cell enumeration and viability measurement.
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perry2021rapidjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Perry, Michele;McDonald, Mary;Lund, Anders;Nikrad, Mrinalini;Wong, Denise;Rice, William L;Chan, Leo Li-Ying;
Journal Journal of microbiological methods
Year 2021
DOI
S0167-7012(21)00249-9
URL
Keywords
viability image cytometry bright field cell counting cellometer x2 e. coli nissle sytox™ green

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