Application of a fluorometric assay for characterization of the catalytic competency of a domain III fragment of Pseudomonas aeruginosa exotoxin A

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ID: 273544
2001
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Abstract
Pseudomonas aeruginosa exotoxin A (ETA) is a member of the family of bacterial ADP-ribosylating toxins that use NAD(+) as the ADP-ribose donor. The reaction catalyzed by ETA involves the nucleophilic attack of the diphthamide residue on the anomeric carbon of the nicotinamide ribose forming a new gl …
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Authors Armstrong S;Merrill AR;;
Journal analytical biochemistry
Year 2001
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/11319814
Keywords
catalysis National Center for Biotechnology Information NCBI NLM MEDLINE pubmed abstract nih national institutes of health national library of medicine reproducibility of results research support non-u.s. gov't Temperature drug combinations osmolar concentration protein structure tertiary peptide fragments / analysis exotoxins / chemistry virulence factors* nad / metabolism pmid:11319814 doi:10.1006/abio.2001.5052 s armstrong a r merrill adp ribose transferases* adenosine diphosphate / metabolism bacterial toxins* exotoxins / analysis* fluorometry / methods* oils peptide fragments / chemistry phenols poly(adp-ribose) polymerases / analysis pseudomonas aeruginosa / chemistry pseudomonas aeruginosa / enzymology*

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