Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4

Clicks: 254
ID: 272625
2004
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Abstract
Laboratory diagnosis of viral respiratory infections is generally performed by virus isolation in cell culture and immunofluorescent assays. Reverse transcriptase PCR is now recognized as a sensitive and specific alternative for detection of respiratory RNA viruses. A rapid real-time multiplex PCR a …
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Authors Templeton KE;Scheltinga SA;Beersma MF;Kroes AC;Claas EC;;
Journal Journal of clinical microbiology
Year 2004
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/15071005
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't cell line sensitivity and specificity Evaluation Study Polymerase Chain Reaction / methods* Respiratory Tract Infections / virology* time factors virus cultivation respiratory syncytial virus infections / virology human / isolation & purification influenza respiratory syncytial virus human / genetics respirovirus infections / diagnosis respiratory tract infections / diagnosis* respiratory syncytial virus infections / diagnosis parainfluenza virus 3 influenza a virus / isolation & purification parainfluenza virus 1 human / diagnosis human / virology influenza a virus / genetics influenza b virus / genetics influenza b virus / isolation & purification pmid:15071005 pmc387552 doi:10.1128/jcm.42.4.1564-1569.2004 kate e templeton sitha a scheltinga eric c j claas parainfluenza virus 2 respirovirus infections / virology rubulavirus infections / diagnosis rubulavirus infections / virology

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