Identification of the sex-determining locus of Atlantic salmon (Salmo salar) on chromosome 2

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ID: 271740
2005
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Abstract
We have integrated data from linkage mapping, physical mapping and karyotyping to gain a better understanding of the sex-determining locus, SEX, in Atlantic salmon (Salmo salar). SEX has been mapped to Atlantic salmon linkage group 1 (ASL1) and is associated with several microsatellite markers. We have used probes designed from the flanking regions of these sex-linked microsatellite markers to screen a bacterial artificial chromosome (BAC) library, representing an 11.7x coverage of the Atlantic salmon genome, which has been HindIII fingerprinted and assembled into contigs. BACs containing sex-linked microsatellites and their related contigs have been identified and representative BACs have been placed on the Atlantic salmon chromosomes by fluorescent in situ hybridization (FISH). This identified chromosome 2, a large metacentric, as the sex chromosome. By positioning several BACs on this chromosome by FISH, it was possible to orient ASL1 with respect to chromosome 2. The region containing SEX appears to lie on the long arm between marker Ssa202DU and a region of heterochromatin identified by DAPI staining. BAC end-sequencing of clones within sex-linked contigs revealed five hitherto unmapped genes along the sex chromosome. We are using an in silico approach coupled with physical probing of the BAC library to extend the BAC contigs to provide a physical map of ASL1, with a view to sequencing chromosome 2 and, in the process, identifying the sex-determining gene.
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Authors C.G. Artieri,Leslie Mitchell,S.H.S. Ng,S.E. Parisotto,R.G. Danzmann,B. Hoyheim,R.B. Phillips,M. Morasch,B.F. Koop,W.S. Davidson;C.G. Artieri;Leslie Mitchell;S.H.S. Ng;S.E. Parisotto;R.G. Danzmann;B. Hoyheim;R.B. Phillips;M. Morasch;B.F. Koop;W.S. Davidson;
Journal cytogenetic and genome research
Year 2005
DOI
10.1159/000087528
URL
https://sciprofiles.com/publication/view/ab9f7a3c1a1d8717f38c52c4835625ff
Keywords
sex microsatellite bac chromosome atlantic salmon physical map asl1 contigs

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