Positional Isomers of Monopegylated Interferon α-2a: Isolation, Characterization, and Biological Activity
Clicks: 203
ID: 270730
1997
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Star Article
30.0
/100
203 views
53 readers
AI Quality Assessment
Not analyzed
Readership in this journal
StarRanked #44 of 75 articles by views in analytical biochemistry
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
The success of recombinant interferon alpha in the clinic in part is limited by two properties of the protein: short serum half-life and immunogenicity. To improve these properties, interferon alpha-2a was conjugated with polyethylene glycol (PEG-5000). A homogeneous preparation of monopegylated interferon alpha-2a was subjected to vigorous analytical and activity characterization. A newly developed ampholyte-free chromatofocussing-like cation-exchange HPLC method utilizing a sulfopropyl resin was used to separate the monopegylated protein into 11 species. Peptide mapping, sequencing, and mass spectrometric analyses indicated that these species are positional isomers where each isomer represents a single polymer molecule conjugated to one specific lysine residue. No species with a modification at the amino terminus was observed. All 11 isomers show antiviral and antiproliferative activities in the same range as the parent monopegylated interferon alpha-2a.
| Reference Key |
pan1997analyticalpositional
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Seth P. Monkarsh,Yuemei Ma,Anthony Aglione,Pascal Bailon,Doreen Ciolek,Barabara Debarbieri,Mary C. Graves,Kurt Hollfelder,Hanspeter Michel,Alicia Palleroni,Jill E. Porter,Emil Russoman,Swapan Roy,Yu-Ching E. Pan;Seth P. Monkarsh;Yuemei Ma;Anthony Aglione;Pascal Bailon;Doreen Ciolek;Barabara Debarbieri;Mary C. Graves;Kurt Hollfelder;Hanspeter Michel;Alicia Palleroni;Jill E. Porter;Emil Russoman;Swapan Roy;Yu-Ching E. Pan; |
| Journal | analytical biochemistry |
| Year | 1997 |
| DOI |
10.1006/abio.1997.2128
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.