Determination of Ephedrine-Type Alkaloids in Dietary Supplements by LC/MS Using a Stable-Isotope Labeled Internal Standard

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2001
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Abstract
Arapid, isocratic method for the separation of 6 ephedrine-type alkaloids which may occur in dietary supplements was modified to permit quantitation and confirmation by liquid chromatography/mass spectrometry using atmospheric pressure chemical ionization. Several columns and sets of conditions were examined for suitability and the results compared. The use of ion-pairing reagents to enhance resolution is also reported. Mass spectrometric conditions were adjusted to provide sufficient fragmentation for qualitative identification of the analytes. Stable-isotope labeled ephedrine was used as an internal standard for quantitation.
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Authors Martha L Gay,Kevin D White,William R Obermeyer,Joseph M Betz,Steven M Musser;Martha L Gay;Kevin D White;William R Obermeyer;Joseph M Betz;Steven M Musser;
Journal journal of aoac international
Year 2001
DOI
10.1093/jaoac/84.3.761
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