Luminol, horseradish peroxidase, and glucose oxidase ternary functionalized graphene oxide for ultrasensitive glucose sensing - Analytical and Bioanalytical Chemistry
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ID: 269592
2017
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Abstract
Luminol, horseradish peroxidase (HRP), and glucose oxidase (GOx) ternary functionalized graphene oxide (HRP/GOx-luminol-GO) with excellent chemiluminescence (CL) activity and specific enzymatic property was prepared via a simple and general strategy for the first time. In this approach, luminol functionalized GO (luminol-GO) was prepared by gently stirring GO with luminol. Then HRP and GOx were further co-immobilized onto the surface of luminol-GO by storing HRP and GOx with luminol-GO at 4 °C overnight, to form HRP/GOx-luminol-GO bionanocomposites. The synthesized HRP/GOx-luminol-GO could react with H2O2 generated from GOx catalyzed glucose oxidization reaction, to produce strong CL emission in the presence of co-immobilized HRP. Thus, we developed an ultrasensitive, homogeneous, reagentless, selective, and simple CL sensing system for glucose detection. The resulting biosensors exhibited ultra-wide linear range from 5.0 nM to 5.0 mM, and an ultra-low detection limit of 1.2 nM, which was more than 3 orders of magnitude lower than previously reported methods. Furthermore, the sensing system was successfully applied for the detection of glucose in human blood samples.
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fang2017analyticalluminol,
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| Authors | Li, Fang;Ma, Wenjing;Liu, Jiachang;Wu, Xiang;Wang, Yan;He, Jianbo;Li, Fang;Ma, Wenjing;Liu, Jiachang;Wu, Xiang;Wang, Yan;He, Jianbo; |
| Journal | Analytical and bioanalytical chemistry |
| Year | 2017 |
| DOI |
doi:10.1007/s00216-017-0752-5
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