Mediatorless amperometric bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase cross-linked to multiwall carbon nanotubes

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ID: 266472
2014
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Abstract
We report on a bienzyme-channeling sensor for sensing glucose without the aid of mediator. It was fabricated by cross-linking horseradish peroxidase (HRP) and glucose oxidase (GOx) on a glassy carbon electrode modified with multiwalled carbon nanotubes (MWNTs). The bienzyme was cross-linked with the MWNTs by glutaraldehyde and bovine serum albumin. The MWNTs were employed to accelerate the electron transfer between immobilized HRP and electrode. Glucose was sensed by amperometric reduction of enzymatically generated H2O2 at an applied voltage of −50 mV (vs. Ag/AgCl). Factors influencing the preparation and performance of the bienzyme electrode were investigated in detail. The biosensor exhibited a fast and linear response to glucose in the concentration range from 0.4 to 15 mM, with a detection limit of 0.4 mM. The sensor exhibited good selectivity and durability, with a long-term relative standard deviation of
Reference Key
xu2014microchimicamediatorless Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Shuxia Xu;Honglan Qi;Shiyi Zhou;Xinfeng Zhang;Chengxiao Zhang;Shuxia Xu;Honglan Qi;Shiyi Zhou;Xinfeng Zhang;Chengxiao Zhang;
Journal microchimica acta
Year 2014
DOI
doi:10.1007/s00604-014-1175-z
URL
https://doi.org/10.1007%2Fs00604-014-1175-z
Keywords
nanotechnology analytical chemistry nanochemistry characterization and evaluation of materials microengineering

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