Mediatorless amperometric bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase cross-linked to multiwall carbon nanotubes
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2014
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Abstract
We report on a bienzyme-channeling sensor for sensing glucose without the aid of mediator. It was fabricated by cross-linking horseradish peroxidase (HRP) and glucose oxidase (GOx) on a glassy carbon electrode modified with multiwalled carbon nanotubes (MWNTs). The bienzyme was cross-linked with the MWNTs by glutaraldehyde and bovine serum albumin. The MWNTs were employed to accelerate the electron transfer between immobilized HRP and electrode. Glucose was sensed by amperometric reduction of enzymatically generated H2O2 at an applied voltage of −50 mV (vs. Ag/AgCl). Factors influencing the preparation and performance of the bienzyme electrode were investigated in detail. The biosensor exhibited a fast and linear response to glucose in the concentration range from 0.4 to 15 mM, with a detection limit of 0.4 mM. The sensor exhibited good selectivity and durability, with a long-term relative standard deviation of
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xu2014microchimicamediatorless
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| Authors | Shuxia Xu;Honglan Qi;Shiyi Zhou;Xinfeng Zhang;Chengxiao Zhang;Shuxia Xu;Honglan Qi;Shiyi Zhou;Xinfeng Zhang;Chengxiao Zhang; |
| Journal | microchimica acta |
| Year | 2014 |
| DOI |
doi:10.1007/s00604-014-1175-z
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