Optimization of the annealing temperature for DNA amplification in vitro.
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ID: 261733
1990
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Abstract
In the polymerase chain reaction (PCR) technique, DNA is amplified in vitro by a series of polymerization cycles consisting of three temperature-dependent steps: DNA denaturation, primer-template annealing, and DNA synthesis by a thermostable DNA polymerase. ...
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| Reference Key |
rhoads1990nucleicoptimization
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|---|---|
| Authors | W Rychlik, W J Spencer, R E Rhoads;W Rychlik;W J Spencer;R E Rhoads; |
| Journal | Nucleic Acids Research |
| Year | 1990 |
| DOI |
10.1093/nar/18.21.6409
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| URL | |
| Keywords |
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