somatic hybridization between citrus sinensis (l.) osbeck and c. grandis (l.) osbeck hibridação somática entre citrus sinensis (l.) osbeck e c. grandis (l.) osbeck

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ID: 260435
2004
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Abstract
This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.
O objetivo deste trabalho foi produzir híbridos somáticos entre laranjas doces e toranjas. Após fusão de protoplastos embriogênicos de laranja doce com protoplastos derivados de mesófilo foliar de toranjas, plantas foram regeneradas por embriogênese somática e aclimatizadas em casa de vegetação. Os híbridos laranja 'Hamlin' + toranja 'Indian Red' e laranja 'Hamlin' + toranja 'Singapura' foram confirmados pela morfologia foliar, contagem do número de cromossomos e análise molecular. Esses híbridos apresentam potencial para serem testados como porta-enxertos tolerantes a declínio, tristeza e doenças causadas por Phytophthora, bem como em programas de melhoramento de porta-enxertos.
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calixto2004pesquisasomatic Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Marcia Cristina Calixto;Francisco de Assis Alves Mourão Filho;Beatriz Madalena Januzzi Mendes;Maria Lúcia Carneiro Vieira
Journal proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006
Year 2004
DOI
10.1590/S0100-204X2004000700015
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