Directed Evolution of a Bacterial Laccase (CueO) for Enzymatic Biofuel Cells.

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ID: 26000
2019
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Abstract
Escherichia coli's copper efflux oxidase (CueO) has rarely been employed in the cathodic compartment of enzymatic biofuel cells (EBFCs) due to its low redox potential (0.36 V vs. Ag/AgCl, pH 5.5) towards O reduction. Herein, directed evolution of CueO towards a more positive onset potential was performed in an electrochemical screening system. An improved CueO variant (D439T/L502K) was obtained with a significantly increased onset potential (0.54 V), comparable to that of high-redox-potential fungal laccases. Upon coupling with an anodic compartment, the EBFC exhibited an open-circuit voltage (V ) of 0.56 V. Directed enzyme evolution by tailoring enzymes to application conditions in EBFCs has been validated and might, in combination with molecular understanding, enable future breakthroughs in EBFC performance.
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zhang2019directedangewandte Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Lingling;Cui, Haiyang;Zou, Zhi;Garakani, Tayebeh Mirzaei;Novoa-Henriquez, Catalina;Jooyeh, Bahareh;Schwaneberg, Ulrich;
Journal angewandte chemie (international ed in english)
Year 2019
DOI
10.1002/anie.201814069
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