Protein-Protected Gold Nanocluster-Based Biosensor for Determining the Prooxidant Activity of Natural Antioxidant Compounds.

Clicks: 187
ID: 25697
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #390 of 475 articles by views in ACS omega

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 475 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
In this work, chicken egg white protein (CEW)-protected gold nanoclusters (CEW-AuNCs) were prepared from CEW and HAuCl to measure the Cu(II)-induced prooxidant activity of antioxidant compounds such as epicatechin, epigallocatechin gallate, catechin, rosmarinic acid, resveratrol, ascorbic acid, and glutathione. These compounds reduced Cu(II) to Cu(I), and the latter was mainly bound to thiol groups in the CEW-AuNC structure. As the protein-bound Cu(I) may act as a catalytic center for generating reactive oxygen species, the Cu(II) reducing ability of antioxidants is an indirect measure of their prooxidant potency. The bound Cu(I) may be released with the cuprous-selective ligand neocuproine (Nc), forming the basis of a spectrophotometric method measuring absorbance at 450 nm wavelength of the Cu(I)-Nc chelate. The developed method involved a one-pot synthesis and determination without preseparation and was applied to binary synthetic mixtures of studied antioxidant compounds and to certain herbal plant (green tea, linden, echinacea, and artichoke leaf) extracts to determine the total prooxidant activities. The obtained results were statistically compared with those of the literature Cu(II)-Nc assay using a calcium proteinate-based solid biosensor. The developed biosensor was durable, reliable, easily applicable, and of low cost and wide linear range and could determine the prooxidant activities of natural antioxidant samples with high reproducibility.
Reference Key
akyuz2019proteinprotectedacs Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Akyüz, Esin;Şen, Furkan Burak;Bener, Mustafa;Başkan, Kevser Sözgen;Tütem, Esma;Apak, Reşat;
Journal ACS omega
Year 2019
DOI
10.1021/acsomega.8b03286
URL
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.