microbiolização e interação entre rizobactérias promotoras do crescimento e clones de eucalipto microbiolization and interaction between growth promoting rhizobacteria and eucalyptus clones
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2009
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Abstract
Os objetivos deste trabalho foram avaliar métodos de microbiolização e estudar a interação entre isolados de rizobactérias e clones de eucalipto. Analisaram-se três métodos de microbiolização: a) Adição de suspensão de rizobactérias a 10(8) ufc/mL no substrato (0,2 mL/cc); b) Imersão de miniestacas na mesma suspensão de inóculo; e c) Combinação dos dois tratamentos (a e b). Empregaram-se um clone híbrido de eucalipto (Eucalyptus grandis x Eucalyptus urophylla) e isolados de rizobactérias (3918, FL2 e S1). Depois de 25 dias do estaqueamento, avaliaram-se o índice de enraizamento, a biomassa radicular e da parte aérea e a incidência de doenças. Em geral, não houve diferença entre os métodos de microbiolização, e todos os isolados aumentaram o enraizamento, a biomassa radicular e o controle biológico de Cylindrocladium spp. Em outro experimento, os resultados indicaram interação entre clones de eucalipto e isolados de rizobactérias para enraizamento e biomassa radicular.
This work aimed to evaluate methods of microbiolization and interaction between isolates of rhizobacteria and eucalyptus clones. We have tested the following methods of microbiolization: a) Addition of suspension of rhizobacteria (10(8) cfu/mL) to the substrate (0.2 mL/cc); b) Immersion of mini-cuttings in the inoculum suspension (10(8) cfu/mL); and c) Combination of the two methods (a and b). Mini-cuttings of a hybrid clone of eucalyptus (Eucalyptus grandis x Eucalyptus urophylla) and three (3918, FL2 and S1) rhizobacteria isolates were used. Twenty-five days after the mini-cuttings were planted, the rooting, root biomass and disease incidence were evaluated. In general, there were no significant differences between the microbiolization methods. All rhizobacterial isolates increased rooting, biomass and biocontrol of Cylindrocladium spp. In another experiment, the results showed interaction between eucalyptus clones and rhizobacteria isolates for the rooting index and root biomass.
This work aimed to evaluate methods of microbiolization and interaction between isolates of rhizobacteria and eucalyptus clones. We have tested the following methods of microbiolization: a) Addition of suspension of rhizobacteria (10(8) cfu/mL) to the substrate (0.2 mL/cc); b) Immersion of mini-cuttings in the inoculum suspension (10(8) cfu/mL); and c) Combination of the two methods (a and b). Mini-cuttings of a hybrid clone of eucalyptus (Eucalyptus grandis x Eucalyptus urophylla) and three (3918, FL2 and S1) rhizobacteria isolates were used. Twenty-five days after the mini-cuttings were planted, the rooting, root biomass and disease incidence were evaluated. In general, there were no significant differences between the microbiolization methods. All rhizobacterial isolates increased rooting, biomass and biocontrol of Cylindrocladium spp. In another experiment, the results showed interaction between eucalyptus clones and rhizobacteria isolates for the rooting index and root biomass.
| Reference Key |
mafia2009revistamicrobiolizao
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| Authors | ;Reginaldo Gonçalves Mafia;Acelino Couto Alfenas;Luiz Antônio Maffia;Eraclides Maria Ferreira;Daniel Henrique Breda Binoti;Leandro Siqueira |
| Journal | journal of veterinary medical research |
| Year | 2009 |
| DOI |
10.1590/S0100-67622009000500002
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