infrared spectroscopy as a tool to study the antioxidant activity of polyphenolic compounds in isolated rat enterocytes
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2016
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Abstract
The protective effect of different polyphenols, catechin (Cat), quercetin (Qc) (flavonoids), gallic acid (GA), caffeic acid (CfA), chlorogenic acid (ChA) (phenolic acids), and capsaicin (Cap), against H2O2-induced oxidative stress was evaluated in rat enterocytes using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Spectroscopy and Fourier Transform Infrared Microspectroscopy (FTIRM), and results were compared to standard lipid peroxidation techniques: conjugated dienes (CD) and Thiobarbituric Acid Reactive Substances (TBARS). Analysis of ATR-FTIR and FTIRM spectral data allowed the simultaneous evaluation of the effects of H2O2 and polyphenols on lipid and protein oxidation. All polyphenols showed a protective effect against H2O2-induced oxidative stress in enterocytes, when administered before or after H2O2. Cat and capsaicin showed the highest protective effect, while phenolic acids had weaker effects and Qc presented a mild prooxidative effect (IR spectral profile of biomolecules between control and H2O2-treated cells) according to FTIR analyses. These results demonstrated the viability to use infrared spectroscopy to evaluate the oxidant and antioxidant effect of molecules in cell systems assays.
| Reference Key |
barraza-garza2016oxidativeinfrared
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|---|---|
| Authors | ;Guillermo Barraza-Garza;Hiram Castillo-Michel;Laura A. de la Rosa;Alejandro Martinez-Martinez;Jorge A. Pérez-León;Marine Cotte;Emilio Alvarez-Parrilla |
| Journal | journal of aoac international |
| Year | 2016 |
| DOI |
10.1155/2016/9245150
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| URL | |
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