evaluation of mycobacterium avium subsp. paratuberculosis faecal culture protocols and media avaliação de protocolos de cultivo fecal e meios para a cultura de mycobacterium avium subsp. paratuberculosis

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ID: 251535
2006
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Abstract
Paratuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map.
A paratuberculose é uma importante enterite de ruminantes causada por Mycobacterium avium subsp. paratuberculosis (Map). A enfermidade é oficialmente considerada exótica no Brasil, mas inquéritos sorológicos recentes e o isolamento do agente etiológico sugerem que ela pode estar presente em nossos rebanhos. O objetivo do estudo foi avaliar três diferentes fórmulas do Ágar gema de ovo de Herrold suplementado com micobactina J (HEYM) e quatro protocolos de cultura fecal quanto ao crescimento de Map, bem como custo e facilidade de implementação. Três fórmulas de HEYM foram inoculadas com duas suspensões de Map. Fezes contaminadas artificialmente e naturalmente com Map foram tratadas pelos quatro protocolos de cultura fecal. O protocolo da centrifugação e a fórmula de HEYM recomendada pela OIE demonstraram os melhores resultados quanto à recuperação de Map.
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ristow2006pesquisaevaluation Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Paula Ristow;Marlei Gomes Silva;Leila de Souza Fonseca;Walter Lilenbaum
Journal AIP Conference Proceedings
Year 2006
DOI
10.1590/S0100-736X2006000100001
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2006000100001
Keywords
diagnosis bovine diagnóstico mycobacterium bovinos paratuberculose mycobacteriumveterinary medicine

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