first report of complete sequence of a blandm-13-harboring plasmid from an escherichia coli st5138 clinical isolate

Clicks: 314
ID: 250653
2016
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #89 of 165 articles by views in electronic physician

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 165 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Since the first report of blaNDM-1, 16 blaNDM variants have been identified among Gram-negative bacteria worldwide. Recently, a novel blaNDM variant, blaNDM-13, was identified in the chromosome of an ST101 Escherichia coli isolate from Nepal. Here we first reported plasmid-mediated blaNDM-13 in a carbapenem-resistant E. coli ST5138 clinical isolate associated with hospital-acquired urinary tract infection from China. blaNDM-13 and blaSHV-12 coexisted on the a ~54 Kb self-transferable plasmid. Compared with NDM-1, NDM-13, NDM-3 and NDM-4 had two amino acid substitutions (D95N and M154L), one amino acid substitution (D95N) and one amino acid substitutions (M154L), respectively. Complete plasmid sequencing showed that blaNDM-13-harboring plasmid (pNDM13-DC33) was highly similar to the blaNDM-1–harboring IncX3 plasmid pNDM-HN380, a common blaNDM-harboring vector circulating in China. In accordance with the structure of pNDM-HN380, pNDM13-DC33 consists of a 33-kb backbone encoding plasmid replication (repB), stability partitioning, and transfer (tra, trb and pil) functions, and a 21-kb antimicrobial resistance region with high GC content between umuD and mpr genes. In conclusion, the present study is the first report of a plasmid-encoded blaNDM-13 and the complete sequence of a blaNDM-13-harboring plasmid (pNDM13-DC33). blaNDM-13 maybe originate from blaNDM-1 located on a pNDM-HN380-like plasmid by sequential mutations.
Reference Key
lv2016frontiersfirst Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Jinnzn Lv, Xiuqing Qi, Dan Zhang, Zhou Zheng, Yuehui Chen, Yunjian Guo, Shanshan Wang, Liang Chen, Barry Kreiswirth, Yiwei Tang, Zengqiang Chen, longhua Hu, Liangxing Wang, Fangyou Yu
Are you one of these authors? Claim your profile
Journal electronic physician
Year 2016
DOI
10.3389/fcimb.2016.00130
URL
http://journal.frontiersin.org/Journal/10.3389/fcimb.2016.00130/full
Keywords
Escherichia coli genome carbapenemase plasmid dna ndm-13microbiology

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.