targeted delivery of gp5 antigen of prrsv to m cells enhances the antigen-specific systemic and mucosal immune responses

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ID: 249929
2018
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Abstract
Efficient delivery of antigens through oral immunization is a first and critical step for successful induction of mucosal immunity, which can provide protection against pathogens invading the mucosa. Membranous/microfold cells (M cells) within the mucosa can transcytose internalized antigen without degradation and thus play an important role in initiating antigen-specific mucosal immune responses through inducing secretory IgA production. In this research, we modified poly (D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) with Ulex europaeus agglutinin 1 (UEA-1) and successfully prepared an oral vaccine delivery system, UEA-1/PLGA NPs. PLGA NPs were prepared using a standard double emulsion solvent evaporation technique, which can protect the entrapped PRRSV DNA vaccine [pcDNA3.1-SynORF5 (synthetic ORF5)] or subunit vaccine ORF5-encoded glycoprotein (GP5) from exposure to the gastrointestinal (GI) tract and release the plasmids in a controlled manner. With UEA-1 modification, the UEA-1/PLGA NPs can be effectively transported by M-cells. We investigated immune response induced by UEA-1/PLGA-SynORF5 or UEA-1/PLGA-GP5 following inoculation in mice and piglets. Compared with PLGA-SynORF5 or PLGA-GP5 NPs, UEA-1/PLGA-SynORF5, or UEA-1/PLGA-GP5 NPs stimulated significantly increased serum IgG levels and augmented intestinal IgA levels in mice and piglets (P < 0.05). Our findings indicate UEA-1/PLGA NPs can be applied as a promising and universally robust oral vaccine delivery system.
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du2018frontierstargeted Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Luping Du;Luping Du;Luping Du;Zhengyu Yu;Zhengyu Yu;Fengjiao Pang;Fengjiao Pang;Xiangwei Xu;Xiangwei Xu;Aihua Mao;Aihua Mao;Wanzhe Yuan;Kongwang He;Kongwang He;Bin Li;Bin Li
Journal electronic physician
Year 2018
DOI
10.3389/fcimb.2018.00007
URL
http://journal.frontiersin.org/article/10.3389/fcimb.2018.00007/full
Keywords
plga immune response prrsv

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