targeted delivery of gp5 antigen of prrsv to m cells enhances the antigen-specific systemic and mucosal immune responses

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ID: 249929
2018
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Abstract
Efficient delivery of antigens through oral immunization is a first and critical step for successful induction of mucosal immunity, which can provide protection against pathogens invading the mucosa. Membranous/microfold cells (M cells) within the mucosa can transcytose internalized antigen without degradation and thus play an important role in initiating antigen-specific mucosal immune responses through inducing secretory IgA production. In this research, we modified poly (D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) with Ulex europaeus agglutinin 1 (UEA-1) and successfully prepared an oral vaccine delivery system, UEA-1/PLGA NPs. PLGA NPs were prepared using a standard double emulsion solvent evaporation technique, which can protect the entrapped PRRSV DNA vaccine [pcDNA3.1-SynORF5 (synthetic ORF5)] or subunit vaccine ORF5-encoded glycoprotein (GP5) from exposure to the gastrointestinal (GI) tract and release the plasmids in a controlled manner. With UEA-1 modification, the UEA-1/PLGA NPs can be effectively transported by M-cells. We investigated immune response induced by UEA-1/PLGA-SynORF5 or UEA-1/PLGA-GP5 following inoculation in mice and piglets. Compared with PLGA-SynORF5 or PLGA-GP5 NPs, UEA-1/PLGA-SynORF5, or UEA-1/PLGA-GP5 NPs stimulated significantly increased serum IgG levels and augmented intestinal IgA levels in mice and piglets (P < 0.05). Our findings indicate UEA-1/PLGA NPs can be applied as a promising and universally robust oral vaccine delivery system.
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du2018frontierstargeted Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Luping Du, Zhengyu Yu, Fengjiao Pang, Xiangwei Xu, Aihua Mao, Wanzhe Yuan, Kongwang He, Bin Li
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Journal electronic physician
Year 2018
DOI
10.3389/fcimb.2018.00007
URL
http://journal.frontiersin.org/article/10.3389/fcimb.2018.00007/full
Keywords
plga immune response prrsv

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