high-throughput microarray for antimicrobial susceptibility testing
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ID: 249807
2017
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Abstract
We describe the development of a novel, high-throughput, nano-scale microarray platform for antimicrobial susceptibility testing (AST). The platform allows to process 480 samples at 50 nL volume on a single chip, analyze by fluorescence read-out with an easy dunk-and-rinse step, and the ability to process multiple samples and chips simultaneously. We demonstrate the applicability of this chip for culturing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA), and perform AST against clinical isolates of CA-MRSA. The chip platform holds promise for an impact in microbial biotechnology as an attractive high-throughput, lower sample volume and quicker alternative to conventional AST such as the traditional broth microdilution or the newer automated systems. Keywords: Antimicrobial susceptibility testing, High-throughput, Microarray
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| Reference Key |
srinivasan2017biotechnologyhigh-throughput
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|---|---|
| Authors | ;Anand Srinivasan;Grace C. Lee;Nelson S. Torres;Kevin Hernandez;Steven D. Dallas;Jose Lopez-Ribot;Christopher R. Frei;Anand K. Ramasubramanian |
| Journal | PLoS genetics |
| Year | 2017 |
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