high-throughput microarray for antimicrobial susceptibility testing

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ID: 249807
2017
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Abstract
We describe the development of a novel, high-throughput, nano-scale microarray platform for antimicrobial susceptibility testing (AST). The platform allows to process 480 samples at 50 nL volume on a single chip, analyze by fluorescence read-out with an easy dunk-and-rinse step, and the ability to process multiple samples and chips simultaneously. We demonstrate the applicability of this chip for culturing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA), and perform AST against clinical isolates of CA-MRSA. The chip platform holds promise for an impact in microbial biotechnology as an attractive high-throughput, lower sample volume and quicker alternative to conventional AST such as the traditional broth microdilution or the newer automated systems. Keywords: Antimicrobial susceptibility testing, High-throughput, Microarray
Reference Key
srinivasan2017biotechnologyhigh-throughput Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Anand Srinivasan, Grace C. Lee, Nelson S. Torres, Kevin Hernandez, Steven D. Dallas, Jose Lopez-Ribot, Christopher R. Frei, Anand K. Ramasubramanian
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Journal PLoS genetics
Year 2017
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2215017X17302576
Keywords
Biotechnology

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