high-throughput discrimination of bacteria isolated from astacus astacus and a. leptodactylus
Clicks: 167
ID: 244500
2014
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Emerging Content
30.0
/100
167 views
24 readers
AI Quality Assessment
Not analyzed
Readership in this journal
EmergingRanked #63 of 125 articles by views in american journal of physiology renal physiology
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 125 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Bacterial diseases and pathogens of crayfish are common, widespread, and occasionally
causing serious mortalities. In order to take rapid measures for correct treatment of
crayfish diseases, the turnover time and accuracy in bacterial identification is an issue.
Bacteria isolated from tissues of apparently healthy Astacus astacus and
A. leptodactylus were identified by the commercial phenotypic tests
(API 20E) and by the matrix assisted laser induced desorption ionization connected to the
time of flight mass spectrometry (MALDI-TOF MS). For Gram-negative rods, API 20E resulted
in fewer species identifications than MALDI-TOF MS (5.2% versus 52.61%). The most
frequently identified genus from A. astacus and A. leptodactylus
was Pseudomonas spp.: API 20E (47.82%) and MALDI-TOF MS
(52.17%). Both systems identified 60.86% of total isolates identically to the genus.
Hafnia alvei was the only isolate for which API 20E and MALDI-TOF MS
had a concordant reading to the species. MALDI-TOF MS proved to be a powerful, low-cost,
rapid tool in bacterial genus identification. This is the first report of a direct
comparison between the two systems for the identification of bacteria in crayfish, and
also the first report on using MALDI-TOF MS for discrimination of freshwater crayfish
bacterial isolates.
| Reference Key |
n.2014knowledgehigh-throughput
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Topić Popović N.;Sauerborn Klobučar R.;Maguire I.;Strunjak-Perović I.;Kazazić S.;Barišić J.;Jadan M.;Klobučar G.;Čož-Rakovac R. |
| Journal | american journal of physiology renal physiology |
| Year | 2014 |
| DOI |
10.1051/kmae/2014005
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.