diversidade genética de pacu do rio paranapanema e do estoque de um programa de repovoamento genetic diversity of pacu from the paranapanema river and from the broodstock of a stock enhancement program

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ID: 240540
2008
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Ranked #30 of 193 articles by views in proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006

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Abstract
O objetivo deste trabalho foi estudar a diversidade genética das amostras de pacu do médio Rio Paranapanema e do estoque de reprodutores utilizado no programa de repovoamento da Estação de Aqüicultura e Hidrologia da usina hidrelétrica Duke Energy, por meio do marcador RAPD. Foram utilizados 14 primers para analisar 30 indivíduos capturados no médio Rio Paranapanema e 29 indivíduos do estoque de reprodutores. O índice de diversidade genética de Shannon e a percentagem de fragmentos polimórficos foram superiores nos indivíduos capturados do Rio Paranapanema. A similaridade genética foi maior nos indivíduos do estoque de reprodutores. A análise da variância molecular mostrou que a maior parte da variação está dentro de cada grupo (84,2%) e não entre os grupos (15,8%). A identidade e distância genética entre os grupos foram 0,9517 e 0,0549, respectivamente. Moderada diferenciação genética (F ST = 0,15) e alto número de migrantes por geração (Nm = 5,33) foram observados entre os dois grupos. Há menor diversidade genética do estoque de reprodutores em relação aos indivíduos capturados do Rio Paranapanema.
The objective of this work was to study the genetic diversity of pacu samples from the middle Paranapanema River and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrology Station at the Duke Energy Power Plant, by RAPD marker. Fourteen primers were used to analyze 30 individuals captured in the middle Paranapanema River and 29 individuals from the broodstock. The Shannon genetic diversity index and percentage of polymorphic fragments were higher in individuals captured in the Paranapanema River. Genetic similarity was larger in individuals of the broodstock. Results of analysis of molecular variance showed that the major part of the genetic variation is within the groups (84.2%) and not between them (15.8%). The identity and genetic distance among the groups were 0.9517 and 0.0549, respectively. Moderate genetic differentiation (F ST = 0.15) and high number of migrants per generation (Nm = 5.33) were observed between the two groups. The genetic diversity was lower in the broodstock than in fish captured in the middle Paranapanema River.
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povh2008pesquisadiversidade Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jayme Aparecido Povh;Ricardo Pereira Ribeiro;Rodolfo Nardez Sirol;Danilo Pedro Streit Júnior;Nelson Mauricio Lopera-Barrero;Lauro Vargas;Patrícia Cristina Gomes;Taís da Silva Lopes
Journal proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006
Year 2008
DOI
10.1590/S0100-204X2008000200007
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000200007
Keywords
rapd molecular markers

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