uso de n-alcanos na estimativa da composição botânica em amostras com diferentes proporções de brachiaria brizantha e arachis pintoi use of n-alkanes for estimations of botanical composition in samples with different proportions of brachiaria brizantha and arachis pintoi

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ID: 237321
2005
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Abstract
Este trabalho foi conduzido para se determinar a composição de n-alcanos (C24 a C36) em diferentes proporções de dietas hipotéticas de Brachiaria brizantha Stapf. cv. Marandu e Arachis pintoi Koprov & Gregory. cv. Amarillo (0; 15; 30; 45; 60 e 100% de Arachis pintoi) e identificar a combinação de alcanos que permite calcular a composição botânica de dietas com o menor valor residual (real menos o estimado). As forragens foram amostradas no verão e os n-alcanos extraídos pelo método de saponificação direta, sendo identificados e quantificados por meio de análise de cromatografia gasosa. O alcano C34 foi utilizado como padrão interno. As proporções de A. pintoi nas dietas foram estimadas pela minimização do z (soma dos quadrados dos desvios) entre a proporção real dos alcanos analisados e as proporções pré-estabelecidas (tratamentos), utilizando-se a equação de Duncan et al. (1999). Observou-se que houve predomínio das cadeias carbônicas ímpares e que a concentração total de n-alcanos decresceu à medida que se aumentou a proporção de A. pintoi nos tratamentos. Estimativas acuradas da composição botânica de misturas de A. pintoi com B. brizantha foram obtidas utilizando-se os alcanos C29, C31, C33 e C35. O alcano C35 foi fundamental para a qualidade das estimativas. Os resultados indicaram o grande potencial da técnica para estudos com animais em pastejo.
This trial was carried out to determine the composition of n-alkanes (C24 to C36) in hypothetical diets comprising of pure Brachiaria brizantha Stapf. cv. Marandu and Arachis pintoi Koprov & Gregory. cv. Amarillo and mixtures of these two spececies with 15%, 30%, 45%, or 60% of Arachis pintoi); it also intended to identify the combination of alkanes that allows to calculate the botanical composition of diets with the smallest residual value (real less estimated values). The forages were sampled in the summer. The n-alkanes were extracted for the direct saponification method and identified and quantified by the gas chromatography analysis. Alkane C34 was used as internal marker. The proportions of A. pintoi in the diets were estimated by the minimization of z (sum of the squares of the deviations) between the real proportion of the analyzed alkanes and the pre-established proportions (treatments), using Duncan et al. (1999) equation. The prevalence of odd carbonic chains was detected. The total concentration of n-alkanes decreased as the proportion of A. pintoi increased in the mixtures. Good estimates of the botanical composition of the mixtures of A. pintoi with B. brizantha were obtained from the alkanes C29, C31, C33 and C35. The alkane C35 was fundamental for the quality of the estimates. The results indicate the great potential of the technique for studies of grazing animal diet.
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crtes2005revistauso Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Cristiano Côrtes;Julio César Damasceno;Rafael Cesário Paine;Nelson Massaru Fukumoto;Fabíola Cristine de Almeida Rêgo;Ulysses Cecato
Journal european journal of lipid science and technology
Year 2005
DOI
10.1590/S1516-35982005000500006
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982005000500006
Keywords
Gas chromatography markers indicadores selectivityanimal culture

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