lessons from in vitro reconstitution analyses of plant microtubule-associated proteins

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ID: 235582
2014
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Abstract
Plant microtubules, composed of tubulin GTPase, are irreplaceable cellular components that regulate the directions of cell expansion and cell division, chromosome segregation and cell plate formation. To accomplish these functions, plant cells organize microtubule structures by regulating microtubule dynamics. Each microtubule localizes to the proper position with repeated growth and shortening. Although it is possible to reconstitute microtubule dynamics with pure tubulin solution in vitro, many microtubule-associated proteins (MAPs) govern microtubule dynamics in cells. In plants, major MAPs are identified as microtubule stabilizers (CLASP and MAP65 etc.), microtubule destabilizers (kinesin-13, katanin, MAP18 and MDP25), and microtubule dynamics promoters (EB1, MAP215, MOR1, MAP200, SPR2). Mutant analyses with forward and reverse genetics have shown the importance of microtubules and individual MAPs in plants. However, it is difficult to understand how each MAP regulates microtubule dynamics, such as growth and shortening, through mutant analyses. In vitro reconstitution analyses with individual purified MAPs and tubulin are powerful tools to reveal how each MAP regulates microtubule dynamics at the molecular level. In this review, I summarize the results of in vitro reconstitution analyses and introduce current models of how each MAP regulates microtubule dynamic instability.
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ehamada2014frontierslessons Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Takahiro eHamada
Journal phytochemistry letters
Year 2014
DOI
10.3389/fpls.2014.00409
URL
http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00409/full
Keywords
plants microtubules arabidopsis tubulin

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