robust Φc31-mediated genome engineering in drosophila melanogaster using minimal attp/attb phage sites

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ID: 232086
2018
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Abstract
Effective genome engineering should lead to a desired locus change with minimal adverse impact to the genome itself. However, flanking loci with site-directed recombinase recognition sites, such as those of the phage ΦC31 integrase, allows for creation of platforms for cassette exchange and manipulation of genomic regions in an iterative manner, once specific loci have been targeted. Here we show that a genomic locus engineered with inverted minimal phage ΦC31 attP/attB sites can undergo efficient recombinase-mediated cassette exchange (RMCE) in the fruit fly Drosophila melanogaster.
Reference Key
voutev2018g3:robust Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Roumen Voutev;Richard S. Mann
Journal separation and purification technology
Year 2018
DOI
10.1534/g3.118.200051
URL
http://g3journal.org/lookup/doi/10.1534/g3.118.200051
Keywords
genome editing crispr/cas9 genome reportgenetics attb/attp sites

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