characterization of thermotolerant chitinases encoded by a brevibacillus laterosporus strain isolated from a suburban wetland

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ID: 230375
2015
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Abstract
To isolate and characterize chitinases that can be applied with practical advantages, 57 isolates of chitin-degrading bacteria were isolated from the soil of a suburban wetland. 16S rRNA gene analysis revealed that the majority of these strains belonged to two genera, Paenibacillus and Brevibacillus. Taking thermostability into account, the chitinases (ChiA and ChiC) of a B. laterosporus strain were studied further. Ni-NTA affinity-purified ChiA and ChiC were optimally active at pH 7.0 and 6.0, respectively, and showed high temperature stability up to 55 °C. Kinetic analysis revealed that ChiC has a lower affinity and stronger catalytic activity toward colloidal chitin than ChiA. With their stability in a broad temperature range, ChiA and ChiC can be utilized for the industrial bioconversion of chitin wastes into biologically active products.
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liu2015genescharacterization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Pulin Liu;Deyong Cheng;Lihong Miao
Journal thermal science and engineering progress
Year 2015
DOI
10.3390/genes6041268
URL
http://www.mdpi.com/2073-4425/6/4/1268
Keywords
kinetic analysis chitinase enzymatic propertiesgenetics

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