new discovery of cryptorchidism: decreased retinoic acid in testicle

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ID: 229997
2016
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Ranked #32 of 144 articles by views in International journal for quality in health care : journal of the International Society for Quality in Health Care

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Abstract
This study focuses on investigation of cryptorchidism induced by flutamide (Flu) and its histopathological damage, and detects retinoic acid concentration in testicle tissue, in order to find a new method for clinical treatment to infertility caused by cryptorchidism. Twenty SD (Sprague Dawley) pregnant rats were randomly divided into Flu cryptorchidism group (n = 10) and normal control group (n = 10). HE stained for observing morphological difference. Transmission electron microscope (TEM) was used for observing the tight junction structure between Sertoli cells. Epididymal caudal sperms were counted and observed in morphology. The expression of stimulated by retinoic acid gene 8 (Stra8) was detected using immunohistochemistry, western blot, and Q-PCR. High performance liquid chromatography (HPLC) analysis was made on retinoic acid content. Sperm count and morphology observation confirmed cryptorchidism group was lower than normal group in sperm quantity and quality. The observation by TEM showed a loose structure of tight junctions between Sertoli cells. Immunohistochemistry, western blot, and Q-PCR showed that cryptorchidism group was significantly lower than normal group in the expression of Stra8. HPLC showed that retinoic acid content was significantly lower in cryptorchid testis than in normal testis. In the cryptorchidism model, retinoic acid content in testicular tissue has a significant reduction; testicles have significant pathological changes; damage exists in the structure of tight junctions between Sertoli cells; Stra8 expression has a significant reduction, perhaps mainly contributing to spermatogenesis disorder.
Reference Key
peng2016saudinew Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jinpu Peng;Lianju Shen;Jinjun Chen;Xining Cao;Yue Zhou;Huali Weng;Chunlan Long;Deying Zhang;Shengfen Tu;Yan Zhang;Dawei He;Tao Lin;Guanghui Wei
Journal International journal for quality in health care : journal of the International Society for Quality in Health Care
Year 2016
DOI
10.1016/j.jsps.2016.04.020
URL
http://www.sciencedirect.com/science/article/pii/S1319016416300214
Keywords
rat cryptorchidism flutamide pharmacology

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