informações fenotípicas e marcadores microssatélites de qtl na escolha de populações segregantes de feijoeiro choice of common bean segregant populations using phenotypic information and qtl microsatellite markers
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2007
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Abstract
O objetivo deste trabalho foi selecionar populações segregantes de feijoeiro promissoras para a produtividade de grãos e com polimorfismo para marcadores microssatélites ligados a QTL relacionados previamente à produtividade de grãos. Foram utilizadas 49 linhagens, avaliadas em dois experimentos em látice triplo. Sete linhagens foram selecionadas e intercruzadas no esquema dialélico e também genotipadas com 24 marcadores microssatélites ligados a QTL previamente identificados. As populações foram avaliadas em blocos completos casualizados, com três repetições. Foram observadas diferenças significativas entre as capacidades gerais (CGC) e específicas de combinação (CEC) e os efeitos não-aditivos foram mais pronunciados. As linhagens RC1-10 e Z-9 se destacaram em razão das elevadas estimativas de g i. Entre os microssatélites, 25% foram polimórficos. Foram selecionadas quatro populações para a seleção de famílias com base na avaliação das linhagens, na análise dialélica e no polimorfismo entre os marcadores microssatélites. Destacou-se a população RC1-10 x Z-9, formada por genitores de alta CGC, com alta média e CEC e ainda grãos do tipo Carioca. Considerando o baixo número de microssatélites polimórficos obtidos nas populações, pode-se concluir haver necessidade de utilização de maior número de microssatélites ligados a QTL da produção de grãos.
The objective of this work was to select promising common bean segregant populations for grain yield and polymorphic to microsatellite markers for grain yield QTL previously identified. Fourty nine lines were utilized and evaluated in two field trials in a triple lattice. Seven lines were selected and intercrossed in a diallel scheme and genotyped with 24 single sequence repeat (SSR) markers associated to grain yield QTL previously identified. Populations were evaluated in a randomized block design with three replications. Significant differences in the general and specific combining ability were observed, and the non-additive effects were highest. The outstanding lines were RC1-10 and Z-9 due to the highest g i values. Twenty five percent of the markers were polymorphic. Four populations were chosen for selecting families based on the line evaluations, the diallel analysis and the polymorphism of SSR markers. Among the segregant populations, RC1-10 x Z-9 is remarkable for having high CEC, high grain yield of the Carioca type, and their parents expressed high CGC. Considering the low number of polymorphic microsatellite markers in each population, it is necessary to use higher number of microsatellite markers associated to grain yield QTL.
The objective of this work was to select promising common bean segregant populations for grain yield and polymorphic to microsatellite markers for grain yield QTL previously identified. Fourty nine lines were utilized and evaluated in two field trials in a triple lattice. Seven lines were selected and intercrossed in a diallel scheme and genotyped with 24 single sequence repeat (SSR) markers associated to grain yield QTL previously identified. Populations were evaluated in a randomized block design with three replications. Significant differences in the general and specific combining ability were observed, and the non-additive effects were highest. The outstanding lines were RC1-10 and Z-9 due to the highest g i values. Twenty five percent of the markers were polymorphic. Four populations were chosen for selecting families based on the line evaluations, the diallel analysis and the polymorphism of SSR markers. Among the segregant populations, RC1-10 x Z-9 is remarkable for having high CEC, high grain yield of the Carioca type, and their parents expressed high CGC. Considering the low number of polymorphic microsatellite markers in each population, it is necessary to use higher number of microsatellite markers associated to grain yield QTL.
| Reference Key |
pereira2007pesquisainformaes
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| Authors | ;Helton Santos Pereira;João Bosco dos Santos;Ângela de Fátima Barbosa Abreu;Karla Rodrigues Couto |
| Journal | proc - the fourth ieee workshop on software technol for future embedded and ubiquitous syst, seus 2006 andthe second int workshop on collaborative comput, integr, and assur, wccia 2006 |
| Year | 2007 |
| DOI |
10.1590/S0100-204X2007000500014
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