electrochemical aptasensor for detecting der p2 allergen using polycarbonate-based double-generation gold nanoparticle chip

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ID: 227116
2017
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Abstract
In this study, a novel aptamer-based impedimetric biosensor for detecting the group 2 allergen of Dermatophagoides pteronyssinus (Der p2) was developed. First, an anodic aluminum oxide (AAO) membrane was prepared. A modified AAO barrier-layer surface with an array of nanohemispheres of 400 nm in diameter was used as a template for the nanoelectroforming of a nickel mold. After electroforming, the AAO template was etched and a nickel nanomold with a concave nanostructure array was produced. The formed nanostructured nickel nanomold was then used in the replica molding of a nanostructured polycarbonate (PC) substrate via hot embossing. Finally, a gold thin film was sputtered onto the PC substrate to form a double-generation gold nanoparticle electrode (array of nanohemispheres with smaller nanoparticles orderly distributed on each nanohemisphere). After immobilizing specifically designed aptamers on the fabricated electrode, electrochemical impedance spectroscopy was used to determine the concentration of Der p2. The sensitivity of the proposed scheme for the detection of the dust mite antigen Der p2 was 2.088 Ω / (ng/mL × cm2) with a dynamic detection range of 27.5–400 ng/mL and detection limit of 16.47 ng/mL.The aptamer-based impedimetric biosensor proposed in this study possesses many advantages such as high sensitivity, low cost, and high consistency over currently used sensors. The proposed sensor was found to be useful for the rapid detection of rare molecules present in an analyte. Keywords: Aptamers, Der p2 dust mite allergen detection, Nanostructured biosensors, Electrochemical impedance spectroscopy
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shen2017sensingelectrochemical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ming-Che Shen;Ji-Ching Lai;Chin-Yih Hong;Gou-Jen Wang
Journal crystals
Year 2017
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2214180416300691
Keywords
engineering (general) civil engineering (general)

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