neural differentiation of adipose-derived stem cells by indirect co-culture with schwann cells

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ID: 226800
2009
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Abstract
To investigate whether adipose-derived stem cells (ADSCs) could be subject to neural differentiation induced only by Schwann cell (SC) factors, we co-cultured ADSCs and SCs in transwell culture dishes. Immunoassaying, Western blot analysis, and RT-PCR were performed (1, 3, 7, 14 d) and the co-cultured ADSCs showed gene and protein expression of S-100, Nestin, and GFAP. Further, qRT-PCR disclosed relative quantitative differences in the above three gene expressions. We think ADSCs can undergo induced neural differentiation by being co-cultured with SCs, and such differentia­tions begin 1 day after co-culture, become apparent after 7 days, and thereafter remain stable till the 14th day.
Reference Key
xiaojie2009archivesneural Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Li Xiaojie;Liao Dapeng;Gong Ping;Yuan Quan;Tan Zhen
Journal gastrointestinal endoscopy
Year 2009
DOI
10.2298/ABS0904703L
URL
http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640904703L.pdf
Keywords
co-culture

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